Visnova’s GLP Compass: Guiding Emerging Pharma Minds from Books to Bench

  Smarter Sample Preparation with dSPE By Visnova Consultancy Introduction In today’s laboratories, sample preparation often consumes more time and resources than the actual analysis. The QuEChERS method—short for Quick, Easy, Cheap, Effective, Rugged, Safe —has revolutionized pesticide residue and contaminant analysis in food, feed, and environmental matrices. What is QuEChERS? QuEChERS is a two-step process: Extraction/Partitioning Sample is extracted with acetonitrile (ACN) , buffered with mild acid, and partitioned using salts such as MgSO₄ and sodium acetate . This step efficiently transfers pesticides and analytes of interest into the ACN phase. Cleanup with dSPE Instead of large SPE cartridges, QuEChERS uses pre-packed centrifuge tubes containing loose sorbents. After vortexing and centrifugation, unwanted co-extractives (pigments, sugars, lipids) bind to the sorbents, leaving a clean extract for LC-MS/MS or GC-MS/MS analysis. Why Miniaturiz...

Partition Coefficient in Agrochemical Pesticides: CIPAC Vs USP Perspective USP Point of View While CIPAC focuses on  pesticides and agrochemical formulations , the  United States Pharmacopeia (USP)  addresses partition coefficient mainly in the context of  pharmaceutical substances . However, the  scientific principles and analytical techniques are nearly identical , which makes the USP perspective valuable when considering pesticides that have  toxicological or pharmacokinetic overlaps . 1. Definition in USP USP refers to the  n-octanol/water partition coefficient  as a measure of a compound’s  lipophilicity  — critical for understanding: Absorption and membrane transport Distribution and tissue affinity Metabolism and excretion The same principles apply to agrochemicals because  environmental fate  is analogous to  drug disposition in the body . 2. USP-Recognized Methods (a) Sh...

  🌿 Determining Partition Coefficient in Agrochemical Pesticides: A CIPAC Perspective Introduction In agrochemical research, understanding how a pesticide behaves in different environments is crucial. One key property that influences its mobility, bioavailability, and potential ecological impact is the partition coefficient (log P) . In this blog, we’ll explore how the partition coefficient is determined in pesticides according to CIPAC (Collaborative International Pesticides Analytical Council) guidelines. What is the Partition Coefficient? The partition coefficient is the ratio of a pesticide’s concentration between two immiscible liquids — typically n-octanol (representing lipids) and water (representing the environment). P = C octanol C water (expressed as log P) P = \frac{C_{\text{octanol}}}{C_{\text{water}}} \quad \text{(expressed as log P)} High log P (>3) → Lipophilic, higher risk of bioaccumulation. Low or negative log P → Hydrophilic, ...

Exploratory Data Analysis in Analytical Chemistry: How YData Profiling Can Transform Your Lab’s Insights By Visnova Consultancy In today’s data-driven laboratories, analytical chemistry is no longer just about instruments and reagents — it’s about making  sense  of the vast amount of data they produce. From chromatographic peak areas to spectroscopic absorbance values, your analytical instruments are generating rich datasets every single day. But here’s the question: Are you extracting every possible insight from that data before making decisions? That’s where  Exploratory Data Analysis (EDA)  and modern tools like  YData Profiling  come in. What is EDA? Exploratory Data Analysis (EDA) is the process of  exploring, summarizing, and visualizing your dataset  to understand its structure, detect patterns, spot anomalies, and prepare it for deeper analysis or modelling. In the context of analytical chemistry, EDA is your  fir...